| Example Program Motif Finder Examples for how to start a motif search using SeqAn's Motif Finder. File "find_motif.cpp" A tutorial about motif search.
1 | #include <iostream>
2 | #include "seqan/find_motif.h"
3 |
4 | using namespace seqan;
5 |
Function to output found motifs.
6 | template <typename TMotifFinder>
7 | void printMotifs(TMotifFinder & finder)
8 | {
9 | for (int i = 0; i < (int) motifCount(finder); ++i)
10 | {
11 | ::std::cout << i << ": " << getMotif(finder, i) << ::std::endl;
12 | }
13 | }
14 |
15 | int main()
16 | {
17 | ::std::srand((unsigned) time(NULL));
18 |
Motif search on a small set of nucleotide sequences.
19 | unsigned int t = 3; //number of input sequences
20 | unsigned int n = 6; //length of sequence
21 | unsigned int l = 4; //length of motif
22 | unsigned int d = 1; //number of substitutions
23 | bool is_exact = true; //size of Hamming distance
24 | unsigned int h = 0; //size of the neighborhood considering at first
25 |
26 | String<DnaString> dataset;
27 | appendValue(dataset,DnaString("ACAGCA"));
28 | appendValue(dataset,DnaString("AGGCAG"));
29 | appendValue(dataset,DnaString("TCAGTC"));
30 |
Application of ePatternBranching (h=0)
31 | MotifFinder<Dna, EPatternBranching> finder_epb1(t,l,d,is_exact,h);
32 | findMotif(finder_epb1,dataset,Omops());
33 | ::std::cout << getMotif(finder_epb1) << ::std::endl;
34 |
Application of ePatternBranching (h=0)
35 | MotifFinder<Dna, EPatternBranching> finder_epb2(t,l,d,is_exact,h);
36 | findMotif(finder_epb2,dataset,Oops());
37 | ::std::cout << getMotif(finder_epb2) << ::std::endl;
38 |
Application of Pms1-Zoops
39 | MotifFinder<Dna, Pms1> finder_pms1(l,d,is_exact);
40 | findMotif(finder_pms1,dataset,Zoops());
41 | printMotifs(finder_pms1);
42 |
Application of Pmsp-Tcm
43 | MotifFinder<Dna, Pmsp> finder_pmsp(l,d,is_exact);
44 | findMotif(finder_pmsp,dataset,Tcm());
45 | printMotifs(finder_pmsp);
46 |
Application of PROJECTION-Oops
47 | unsigned int m = t*(n-l+1);
48 | MotifFinder<Dna, Projection> finder_proj(t,l,m,d,is_exact);
49 | findMotif(finder_proj, dataset, Oops());
50 | printMotifs(finder_proj);
51 |
Application of PROJECTION-Omops
52 | MotifFinder<Dna, Projection> finder_proj_omops(t,l,m,d,is_exact);
53 | findMotif(finder_proj_omops, dataset, Omops());
54 | printMotifs(finder_proj_omops);
55 |
Application of PROJECTION-Zoops
56 | MotifFinder<Dna, Projection> finder_proj_zoops(t,l,m,d,is_exact);
57 | findMotif(finder_proj_zoops, dataset, Zoops());
58 | printMotifs(finder_proj_zoops);
59 |
Application of PROJECTION-Tcm
60 | MotifFinder<Dna, Projection> finder_proj_tcm(t,l,m,d,is_exact);
61 | findMotif(finder_proj_tcm, dataset, Tcm());
62 | printMotifs(finder_proj_tcm);
63 |
64 | return 0;
65 | }
66 |
Output [0]: AGCC [0]: AGCC [0]: AAGC [1]: ACAG [2]: AGAC [3]: AGCC [4]: AGGA [5]: AGTA [6]: CAGA [7]: CAGG [8]: CCAG [9]: CGCA [10]: CGGC [11]: GCAG [12]: TCAG [13]: TGCA [0]: AAGC [1]: AAGT [2]: AATC [3]: ACAG [4]: ACGC [5]: ACTC [6]: AGAC [7]: AGCA [8]: AGCC [9]: AGGA [10]: AGGC [11]: AGGG [12]: AGGT [13]: AGTA [14]: AGTC [15]: AGTG [16]: AGTT [17]: ATGC [18]: ATTC [19]: CAAT [20]: CACT [21]: CAGA [22]: CAGC [23]: CAGG [24]: CATT [25]: CCAG [26]: CCGT [27]: CGCA [28]: CGGC [29]: CGGT [30]: CGTC [31]: CTGT [32]: GAAG [33]: GACA [34]: GAGT [35]: GCAA [36]: GCAC [37]: GCAG [38]: GCAT [39]: GCCA [40]: GCCG [41]: GCGG [42]: GCTG [43]: GGAA [44]: GGAG [45]: GGCC [46]: GGCG [47]: GGCT [48]: GGGA [49]: GGGC [50]: GGTA [51]: GGTC [52]: GTAG [53]: GTCA [54]: TAAG [55]: TAGT [56]: TCAA [57]: TCAC [58]: TCAG [59]: TCAT [60]: TCCG [61]: TCGG [62]: TCTG [63]: TGAG [64]: TGCA [65]: TGGC [66]: TGTC [67]: TTAG AGCC AGCC TCAG TCAG See MotifFinder, findMotif SeqAn - Sequence Analysis Library - www.seqan.de

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